Construct: shRNA TRCN0000307210

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145230.2-1239s21c1
DNA Barcode:
CTGTTACCTCTTCTGGCTCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATP6V0E2 (155066)
Gene Description:
ATPase H+ transporting V0 subunit e2
Transcript:
RefSeq NM_145230.2 (NON-CURRENT)
Match location:
Position 1239 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000307210)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGTTACCTCTTCTGGCTCAT
Hairpin Sequence:
5'-CCGG-CTGTTACCTCTTCTGGCTCAT-CTCGAG-ATGAGCCAGAAGAGGTAACAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGTTACCTCTTCTGGCTCATCTCGAGATGAGCCAGAAGAGGTAACAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGTTACCTCTTCTGGCTCATCTCGAGATGAGCCAGAAGAGGTAACAG-3'

Other clones with same target sequence:

  1. TRCN0000082737