Construct: shRNA TRCN0000307239

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005909.3-7068s21c1
DNA Barcode:
GCTTGAAAGAATCCTCGGATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAP1B (4131)
Gene Description:
microtubule associated protein 1B
Transcript:
RefSeq NM_005909.3 (NON-CURRENT)
Match location:
Position 7068 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000307239)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTGAAAGAATCCTCGGATA
Hairpin Sequence:
5'-CCGG-GCTTGAAAGAATCCTCGGATA-CTCGAG-TATCCGAGGATTCTTTCAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTGAAAGAATCCTCGGATACTCGAGTATCCGAGGATTCTTTCAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTGAAAGAATCCTCGGATACTCGAGTATCCGAGGATTCTTTCAAGC-3'

Other clones with same target sequence:

  1. TRCN0000116619