Construct: shRNA TRCN0000307419

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018768.2-1227s21c1
DNA Barcode:
CCCAACAGTGCTGGCTATTTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Stx8 (55943)
Gene Description:
syntaxin 8
Transcript:
RefSeq NM_018768.2 (CURRENT)
Match location:
Position 1227 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000307419)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAACAGTGCTGGCTATTTC
Hairpin Sequence:
5'-CCGG-CCCAACAGTGCTGGCTATTTC-CTCGAG-GAAATAGCCAGCACTGTTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAACAGTGCTGGCTATTTCCTCGAGGAAATAGCCAGCACTGTTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAACAGTGCTGGCTATTTCCTCGAGGAAATAGCCAGCACTGTTGGG-3'

Other clones with same target sequence:

(none)