Construct: shRNA TRCN0000307435

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007839.2-2738s21c1
DNA Barcode:
TGTTGACACTGTAGCTCATAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dhx15 (13204)
Gene Description:
DEAH (Asp-Glu-Ala-His) box polypeptide 15
Transcript:
RefSeq NM_007839.2 (NON-CURRENT)
Match location:
Position 2738 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000307435)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGTTGACACTGTAGCTCATAC
Hairpin Sequence:
5'-CCGG-TGTTGACACTGTAGCTCATAC-CTCGAG-GTATGAGCTACAGTGTCAACA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTTGACACTGTAGCTCATACCTCGAGGTATGAGCTACAGTGTCAACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTTGACACTGTAGCTCATACCTCGAGGTATGAGCTACAGTGTCAACA-3'

Other clones with same target sequence:

(none)