Construct: shRNA TRCN0000307495

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009980.4-1607s21c1
DNA Barcode:
TACGAAACTGTGTCAACAAAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ctbp2 (13017)
Gene Description:
C-terminal binding protein 2
Transcript:
RefSeq NM_009980.4 (CURRENT)
Match location:
Position 1607 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000307495)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TACGAAACTGTGTCAACAAAG
Hairpin Sequence:
5'-CCGG-TACGAAACTGTGTCAACAAAG-CTCGAG-CTTTGTTGACACAGTTTCGTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTACGAAACTGTGTCAACAAAGCTCGAGCTTTGTTGACACAGTTTCGTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATACGAAACTGTGTCAACAAAGCTCGAGCTTTGTTGACACAGTTTCGTA-3'

Other clones with same target sequence:

(none)