Construct: shRNA TRCN0000307627

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009462.1-2353s21c1
DNA Barcode:
CCAAACGGTCAAGGTTATTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Usp10 (22224)
Gene Description:
ubiquitin specific peptidase 10
Transcript:
RefSeq NM_009462.1 (NON-CURRENT)
Match location:
Position 2353 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000307627)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAACGGTCAAGGTTATTAA
Hairpin Sequence:
5'-CCGG-CCAAACGGTCAAGGTTATTAA-CTCGAG-TTAATAACCTTGACCGTTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAACGGTCAAGGTTATTAACTCGAGTTAATAACCTTGACCGTTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAACGGTCAAGGTTATTAACTCGAGTTAATAACCTTGACCGTTTGG-3'

Other clones with same target sequence:

(none)