Construct: shRNA TRCN0000307917

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003757.2-984s21c1
DNA Barcode:
CGAAGATGGTTACGTCCGTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EIF3I (8668)
Gene Description:
eukaryotic translation initiation factor 3 subunit I
Transcript:
RefSeq NM_003757.2 (NON-CURRENT)
Match location:
Position 984 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000307917)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAAGATGGTTACGTCCGTAT
Hairpin Sequence:
5'-CCGG-CGAAGATGGTTACGTCCGTAT-CTCGAG-ATACGGACGTAACCATCTTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAAGATGGTTACGTCCGTATCTCGAGATACGGACGTAACCATCTTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAAGATGGTTACGTCCGTATCTCGAGATACGGACGTAACCATCTTCG-3'

Other clones with same target sequence:

  1. TRCN0000074817