Construct: shRNA TRCN0000308123

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177925.2-227s21c1
DNA Barcode:
TGCACAGACTGCTGCGCAAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
H2AJ (55766)
Gene Description:
H2A.J histone
Transcript:
RefSeq NM_177925.2 (NON-CURRENT)
Match location:
Position 227 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000308123)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCACAGACTGCTGCGCAAAG
Hairpin Sequence:
5'-CCGG-TGCACAGACTGCTGCGCAAAG-CTCGAG-CTTTGCGCAGCAGTCTGTGCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCACAGACTGCTGCGCAAAGCTCGAGCTTTGCGCAGCAGTCTGTGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCACAGACTGCTGCGCAAAGCTCGAGCTTTGCGCAGCAGTCTGTGCA-3'

Other clones with same target sequence:

(none)