Construct: shRNA TRCN0000308224

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001425.2-460s21c1
DNA Barcode:
TCATGGTGCTCTCCCTCATTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EMP3 (2014)
Gene Description:
epithelial membrane protein 3
Transcript:
RefSeq NM_001425.2 (NON-CURRENT)
Match location:
Position 460 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000308224)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCATGGTGCTCTCCCTCATTC
Hairpin Sequence:
5'-CCGG-TCATGGTGCTCTCCCTCATTC-CTCGAG-GAATGAGGGAGAGCACCATGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCATGGTGCTCTCCCTCATTCCTCGAGGAATGAGGGAGAGCACCATGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCATGGTGCTCTCCCTCATTCCTCGAGGAATGAGGGAGAGCACCATGA-3'

Other clones with same target sequence:

(none)