Construct: shRNA TRCN0000308364

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008163.3-723s21c1
DNA Barcode:
TGGTAGATTACCACAGATCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Grb2 (14784)
Gene Description:
growth factor receptor bound protein 2
Transcript:
RefSeq NM_008163.3 (NON-CURRENT)
Match location:
Position 723 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000308364)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGTAGATTACCACAGATCAA
Hairpin Sequence:
5'-CCGG-TGGTAGATTACCACAGATCAA-CTCGAG-TTGATCTGTGGTAATCTACCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGTAGATTACCACAGATCAACTCGAGTTGATCTGTGGTAATCTACCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGTAGATTACCACAGATCAACTCGAGTTGATCTGTGGTAATCTACCA-3'

Other clones with same target sequence:

  1. TRCN0000097111