Construct: shRNA TRCN0000308429

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144892.1-649s21c1
DNA Barcode:
CGACGTAGAGAGGAGCTTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ncoa5 (228869)
Gene Description:
nuclear receptor coactivator 5
Transcript:
RefSeq NM_144892.1 (CURRENT)
Match location:
Position 649 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000308429)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGACGTAGAGAGGAGCTTTAT
Hairpin Sequence:
5'-CCGG-CGACGTAGAGAGGAGCTTTAT-CTCGAG-ATAAAGCTCCTCTCTACGTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGACGTAGAGAGGAGCTTTATCTCGAGATAAAGCTCCTCTCTACGTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGACGTAGAGAGGAGCTTTATCTCGAGATAAAGCTCCTCTCTACGTCG-3'

Other clones with same target sequence:

  1. TRCN0000126152