Construct: shRNA TRCN0000308460

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009955.3-1862s21c1
DNA Barcode:
GCTCAGATTGACGACAACATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dpysl2 (12934)
Gene Description:
dihydropyrimidinase-like 2
Transcript:
RefSeq NM_009955.3 (CURRENT)
Match location:
Position 1862 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000308460)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCAGATTGACGACAACATT
Hairpin Sequence:
5'-CCGG-GCTCAGATTGACGACAACATT-CTCGAG-AATGTTGTCGTCAATCTGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCAGATTGACGACAACATTCTCGAGAATGTTGTCGTCAATCTGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCAGATTGACGACAACATTCTCGAGAATGTTGTCGTCAATCTGAGC-3'

Other clones with same target sequence:

  1. TRCN0000032640