Construct: shRNA TRCN0000308469

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009468.4-886s21c1
DNA Barcode:
GCTCAAGTTCATGCCGAGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dpysl3 (22240)
Gene Description:
dihydropyrimidinase-like 3
Transcript:
RefSeq NM_009468.4 (NON-CURRENT)
Match location:
Position 886 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000308469)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCAAGTTCATGCCGAGAAT
Hairpin Sequence:
5'-CCGG-GCTCAAGTTCATGCCGAGAAT-CTCGAG-ATTCTCGGCATGAACTTGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCAAGTTCATGCCGAGAATCTCGAGATTCTCGGCATGAACTTGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCAAGTTCATGCCGAGAATCTCGAGATTCTCGGCATGAACTTGAGC-3'

Other clones with same target sequence:

  1. TRCN0000032670