Construct: shRNA TRCN0000308471

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011780.2-2336s21c1
DNA Barcode:
CCGACTTCCTTCTATCATCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Adam23 (23792)
Gene Description:
a disintegrin and metallopeptidase domain 23
Transcript:
RefSeq NM_011780.2 (NON-CURRENT)
Match location:
Position 2336 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000308471)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGACTTCCTTCTATCATCAA
Hairpin Sequence:
5'-CCGG-CCGACTTCCTTCTATCATCAA-CTCGAG-TTGATGATAGAAGGAAGTCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGACTTCCTTCTATCATCAACTCGAGTTGATGATAGAAGGAAGTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGACTTCCTTCTATCATCAACTCGAGTTGATGATAGAAGGAAGTCGG-3'

Other clones with same target sequence:

  1. TRCN0000032662