Construct: shRNA TRCN0000308511

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138600.4-962s21c1
DNA Barcode:
GCTTGGAGGAAACAATGCCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Aldh7a1 (110695)
Gene Description:
aldehyde dehydrogenase family 7, member A1
Transcript:
RefSeq NM_138600.4 (CURRENT)
Match location:
Position 962 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000308511)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTGGAGGAAACAATGCCAT
Hairpin Sequence:
5'-CCGG-GCTTGGAGGAAACAATGCCAT-CTCGAG-ATGGCATTGTTTCCTCCAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTGGAGGAAACAATGCCATCTCGAGATGGCATTGTTTCCTCCAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTGGAGGAAACAATGCCATCTCGAGATGGCATTGTTTCCTCCAAGC-3'

Other clones with same target sequence:

  1. TRCN0000042077