Construct: shRNA TRCN0000308653

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007437.4-851s21c1
DNA Barcode:
CCTGACTATATCCTGTGCGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Aldh3a2 (11671)
Gene Description:
aldehyde dehydrogenase family 3, subfamily A2
Transcript:
RefSeq NM_007437.4 (NON-CURRENT)
Match location:
Position 851 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000308653)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGACTATATCCTGTGCGAA
Hairpin Sequence:
5'-CCGG-CCTGACTATATCCTGTGCGAA-CTCGAG-TTCGCACAGGATATAGTCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGACTATATCCTGTGCGAACTCGAGTTCGCACAGGATATAGTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGACTATATCCTGTGCGAACTCGAGTTCGCACAGGATATAGTCAGG-3'

Other clones with same target sequence:

  1. TRCN0000041372