Construct: shRNA TRCN0000308861

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026440.3-935s21c1
DNA Barcode:
GCGGATACGATGCTTAGAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rnmt (67897)
Gene Description:
RNA (guanine-7-) methyltransferase
Transcript:
RefSeq NM_026440.3 (NON-CURRENT)
Match location:
Position 935 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000308861)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGGATACGATGCTTAGAAAT
Hairpin Sequence:
5'-CCGG-GCGGATACGATGCTTAGAAAT-CTCGAG-ATTTCTAAGCATCGTATCCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGGATACGATGCTTAGAAATCTCGAGATTTCTAAGCATCGTATCCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGGATACGATGCTTAGAAATCTCGAGATTTCTAAGCATCGTATCCGC-3'

Other clones with same target sequence:

  1. TRCN0000039145