Construct: shRNA TRCN0000308892

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025303.3-1607s21c1
DNA Barcode:
GCCAGGGAACTCCTTATGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Stau2 (29819)
Gene Description:
staufen (RNA binding protein) homolog 2 (Drosophila)
Transcript:
RefSeq NM_025303.3 (CURRENT)
Match location:
Position 1607 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000308892)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAGGGAACTCCTTATGAAT
Hairpin Sequence:
5'-CCGG-GCCAGGGAACTCCTTATGAAT-CTCGAG-ATTCATAAGGAGTTCCCTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAGGGAACTCCTTATGAATCTCGAGATTCATAAGGAGTTCCCTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAGGGAACTCCTTATGAATCTCGAGATTCATAAGGAGTTCCCTGGC-3'

Other clones with same target sequence:

  1. TRCN0000102356
  2. TRCN0000157149