Construct: shRNA TRCN0000308935

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009755.2-572s21c1
DNA Barcode:
CCGTTTGTGATTGGAGGGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Bmp1 (12153)
Gene Description:
bone morphogenetic protein 1
Transcript:
RefSeq NM_009755.2 (NON-CURRENT)
Match location:
Position 572 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000308935)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGTTTGTGATTGGAGGGAAT
Hairpin Sequence:
5'-CCGG-CCGTTTGTGATTGGAGGGAAT-CTCGAG-ATTCCCTCCAATCACAAACGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGTTTGTGATTGGAGGGAATCTCGAGATTCCCTCCAATCACAAACGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGTTTGTGATTGGAGGGAATCTCGAGATTCCCTCCAATCACAAACGG-3'

Other clones with same target sequence:

  1. TRCN0000031375