Construct: shRNA TRCN0000308960
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_023172.3-353s21c1
- DNA Barcode:
- CCCTCTGAGAAAGCAATGTAT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Ndufb9 (66218)
- Gene Description:
- NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9
- Transcript:
- RefSeq NM_023172.3 (CURRENT)
- Match location:
- Position 353 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000308960)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCCTCTGAGAAAGCAATGTAT
- Hairpin Sequence:
-
5'-CCGG-CCCTCTGAGAAAGCAATGTAT-CTCGAG-ATACATTGCTTTCTCAGAGGG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCCTCTGAGAAAGCAATGTATCTCGAGATACATTGCTTTCTCAGAGGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCCTCTGAGAAAGCAATGTATCTCGAGATACATTGCTTTCTCAGAGGG-3'
Other clones with same target sequence:
-
TRCN0000041560
