Construct: shRNA TRCN0000309119

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008775.3-366s21c1
DNA Barcode:
CCCACTTCATGCTCTTAATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pafah1b2 (18475)
Gene Description:
platelet-activating factor acetylhydrolase, isoform 1b, subunit 2
Transcript:
RefSeq NM_008775.3 (CURRENT)
Match location:
Position 366 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000309119)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCACTTCATGCTCTTAATTT
Hairpin Sequence:
5'-CCGG-CCCACTTCATGCTCTTAATTT-CTCGAG-AAATTAAGAGCATGAAGTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCACTTCATGCTCTTAATTTCTCGAGAAATTAAGAGCATGAAGTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCACTTCATGCTCTTAATTTCTCGAGAAATTAAGAGCATGAAGTGGG-3'

Other clones with same target sequence:

  1. TRCN0000098231