Construct: shRNA TRCN0000309331

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138651.6-3720s21c1
DNA Barcode:
GCGTGTTGTTTGTTCCAGTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cds2 (110911)
Gene Description:
CDP-diacylglycerol synthase (phosphatidate cytidylyltransferase) 2
Transcript:
RefSeq NM_138651.6 (NON-CURRENT)
Match location:
Position 3720 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000309331)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGTGTTGTTTGTTCCAGTAA
Hairpin Sequence:
5'-CCGG-GCGTGTTGTTTGTTCCAGTAA-CTCGAG-TTACTGGAACAAACAACACGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGTGTTGTTTGTTCCAGTAACTCGAGTTACTGGAACAAACAACACGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGTGTTGTTTGTTCCAGTAACTCGAGTTACTGGAACAAACAACACGC-3'

Other clones with same target sequence:

  1. TRCN0000076243