Construct: shRNA TRCN0000309344

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021393.2-2306s21c1
DNA Barcode:
CCTGATATTCAATCTGCACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nelfb (58202)
Gene Description:
negative elongation factor complex member B
Transcript:
RefSeq NM_021393.2 (NON-CURRENT)
Match location:
Position 2306 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000309344)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGATATTCAATCTGCACAA
Hairpin Sequence:
5'-CCGG-CCTGATATTCAATCTGCACAA-CTCGAG-TTGTGCAGATTGAATATCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGATATTCAATCTGCACAACTCGAGTTGTGCAGATTGAATATCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGATATTCAATCTGCACAACTCGAGTTGTGCAGATTGAATATCAGG-3'

Other clones with same target sequence:

  1. TRCN0000126519