Construct: shRNA TRCN0000309417

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144822.2-866s21c1
DNA Barcode:
CCCTCAAGTCTGGCTTATGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Micu1 (216001)
Gene Description:
mitochondrial calcium uptake 1
Transcript:
RefSeq NM_144822.2 (NON-CURRENT)
Match location:
Position 866 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000309417)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTCAAGTCTGGCTTATGTT
Hairpin Sequence:
5'-CCGG-CCCTCAAGTCTGGCTTATGTT-CTCGAG-AACATAAGCCAGACTTGAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTCAAGTCTGGCTTATGTTCTCGAGAACATAAGCCAGACTTGAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTCAAGTCTGGCTTATGTTCTCGAGAACATAAGCCAGACTTGAGGG-3'

Other clones with same target sequence:

  1. TRCN0000104656