Construct: shRNA TRCN0000309481

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016773.3-1083s21c1
DNA Barcode:
AGCACTATTCACAAGAGAGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nucb2 (53322)
Gene Description:
nucleobindin 2
Transcript:
RefSeq NM_016773.3 (NON-CURRENT)
Match location:
Position 1083 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000309481)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCACTATTCACAAGAGAGTT
Hairpin Sequence:
5'-CCGG-AGCACTATTCACAAGAGAGTT-CTCGAG-AACTCTCTTGTGAATAGTGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCACTATTCACAAGAGAGTTCTCGAGAACTCTCTTGTGAATAGTGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCACTATTCACAAGAGAGTTCTCGAGAACTCTCTTGTGAATAGTGCT-3'

Other clones with same target sequence:

  1. TRCN0000104609