Construct: shRNA TRCN0000309516

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009682.3-292s21c1
DNA Barcode:
GTGGATTCATCAGAGAGTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ap3s2 (11778)
Gene Description:
adaptor-related protein complex 3, sigma 2 subunit
Transcript:
RefSeq NM_009682.3 (CURRENT)
Match location:
Position 292 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000309516)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGGATTCATCAGAGAGTGAA
Hairpin Sequence:
5'-CCGG-GTGGATTCATCAGAGAGTGAA-CTCGAG-TTCACTCTCTGATGAATCCAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGGATTCATCAGAGAGTGAACTCGAGTTCACTCTCTGATGAATCCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGGATTCATCAGAGAGTGAACTCGAGTTCACTCTCTGATGAATCCAC-3'

Other clones with same target sequence:

  1. TRCN0000113174