Construct: shRNA TRCN0000309530

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026703.2-254s21c1
DNA Barcode:
GAACTTCTTCAGGCAGATAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ndufa8 (68375)
Gene Description:
NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 8
Transcript:
RefSeq NM_026703.2 (CURRENT)
Match location:
Position 254 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000309530)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACTTCTTCAGGCAGATAAA
Hairpin Sequence:
5'-CCGG-GAACTTCTTCAGGCAGATAAA-CTCGAG-TTTATCTGCCTGAAGAAGTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACTTCTTCAGGCAGATAAACTCGAGTTTATCTGCCTGAAGAAGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACTTCTTCAGGCAGATAAACTCGAGTTTATCTGCCTGAAGAAGTTC-3'

Other clones with same target sequence:

  1. TRCN0000041840