Construct: shRNA TRCN0000309676

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133815.1-2348s21c1
DNA Barcode:
GCTGAAGAATAAGGGCAGATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lbr (98386)
Gene Description:
lamin B receptor
Transcript:
RefSeq NM_133815.1 (NON-CURRENT)
Match location:
Position 2348 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000309676)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGAAGAATAAGGGCAGATT
Hairpin Sequence:
5'-CCGG-GCTGAAGAATAAGGGCAGATT-CTCGAG-AATCTGCCCTTATTCTTCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGAAGAATAAGGGCAGATTCTCGAGAATCTGCCCTTATTCTTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGAAGAATAAGGGCAGATTCTCGAGAATCTGCCCTTATTCTTCAGC-3'

Other clones with same target sequence:

  1. TRCN0000054903