Construct: shRNA TRCN0000309686

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019713.3-640s21c1
DNA Barcode:
GCACTCTTTGAGCGAACTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rassf1 (56289)
Gene Description:
Ras association (RalGDS/AF-6) domain family member 1
Transcript:
RefSeq NM_019713.3 (NON-CURRENT)
Match location:
Position 640 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000309686)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACTCTTTGAGCGAACTGAA
Hairpin Sequence:
5'-CCGG-GCACTCTTTGAGCGAACTGAA-CTCGAG-TTCAGTTCGCTCAAAGAGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACTCTTTGAGCGAACTGAACTCGAGTTCAGTTCGCTCAAAGAGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACTCTTTGAGCGAACTGAACTCGAGTTCAGTTCGCTCAAAGAGTGC-3'

Other clones with same target sequence:

  1. TRCN0000054969