Construct: shRNA TRCN0000309739

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146217.3-1699s21c1
DNA Barcode:
GACCTCATCATGCTGGACATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Aars (234734)
Gene Description:
alanyl-tRNA synthetase
Transcript:
RefSeq NM_146217.3 (NON-CURRENT)
Match location:
Position 1699 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000309739)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACCTCATCATGCTGGACATT
Hairpin Sequence:
5'-CCGG-GACCTCATCATGCTGGACATT-CTCGAG-AATGTCCAGCATGATGAGGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACCTCATCATGCTGGACATTCTCGAGAATGTCCAGCATGATGAGGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACCTCATCATGCTGGACATTCTCGAGAATGTCCAGCATGATGAGGTC-3'

Other clones with same target sequence:

  1. TRCN0000075967