Construct: shRNA TRCN0000309875

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013657.5-681s21c1
DNA Barcode:
CGATGCTCTTTCAACCCGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sema3c (20348)
Gene Description:
sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3C
Transcript:
RefSeq NM_013657.5 (CURRENT)
Match location:
Position 681 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000309875)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGATGCTCTTTCAACCCGAAT
Hairpin Sequence:
5'-CCGG-CGATGCTCTTTCAACCCGAAT-CTCGAG-ATTCGGGTTGAAAGAGCATCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGATGCTCTTTCAACCCGAATCTCGAGATTCGGGTTGAAAGAGCATCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGATGCTCTTTCAACCCGAATCTCGAGATTCGGGTTGAAAGAGCATCG-3'

Other clones with same target sequence:

  1. TRCN0000067391