Construct: shRNA TRCN0000310077

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025065.6-197s21c1
DNA Barcode:
GCTTTAGCATTTCGGAGATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RPF1 (80135)
Gene Description:
ribosome production factor 1 homolog
Transcript:
RefSeq NM_025065.6 (NON-CURRENT)
Match location:
Position 197 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000310077)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTTAGCATTTCGGAGATTA
Hairpin Sequence:
5'-CCGG-GCTTTAGCATTTCGGAGATTA-CTCGAG-TAATCTCCGAAATGCTAAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTTAGCATTTCGGAGATTACTCGAGTAATCTCCGAAATGCTAAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTTAGCATTTCGGAGATTACTCGAGTAATCTCCGAAATGCTAAAGC-3'

Other clones with same target sequence:

  1. TRCN0000151572