Construct: shRNA TRCN0000310092

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004225.2-3193s21c1
DNA Barcode:
CTGAGCAGTTGCAGATTGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MFHAS1 (9258)
Gene Description:
malignant fibrous histiocytoma amplified sequence 1
Transcript:
RefSeq NM_004225.2 (NON-CURRENT)
Match location:
Position 3193 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000310092)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGAGCAGTTGCAGATTGAAT
Hairpin Sequence:
5'-CCGG-CTGAGCAGTTGCAGATTGAAT-CTCGAG-ATTCAATCTGCAACTGCTCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGAGCAGTTGCAGATTGAATCTCGAGATTCAATCTGCAACTGCTCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGAGCAGTTGCAGATTGAATCTCGAGATTCAATCTGCAACTGCTCAG-3'

Other clones with same target sequence:

  1. TRCN0000073032