Construct: shRNA TRCN0000310240

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001344.2-162s21c1
DNA Barcode:
GACGCGTACCTGCTGTATATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DAD1 (1603)
Gene Description:
defender against cell death 1
Transcript:
RefSeq NM_001344.2 (NON-CURRENT)
Match location:
Position 162 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000310240)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACGCGTACCTGCTGTATATA
Hairpin Sequence:
5'-CCGG-GACGCGTACCTGCTGTATATA-CTCGAG-TATATACAGCAGGTACGCGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACGCGTACCTGCTGTATATACTCGAGTATATACAGCAGGTACGCGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACGCGTACCTGCTGTATATACTCGAGTATATACAGCAGGTACGCGTC-3'

Other clones with same target sequence:

(none)