Construct: shRNA TRCN0000310510

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004318.3-436s21c1
DNA Barcode:
CGTGGTTTATGGTGATTGCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ASPH (444)
Gene Description:
aspartate beta-hydroxylase
Transcript:
RefSeq NM_004318.3 (NON-CURRENT)
Match location:
Position 436 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000310510)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTGGTTTATGGTGATTGCAT
Hairpin Sequence:
5'-CCGG-CGTGGTTTATGGTGATTGCAT-CTCGAG-ATGCAATCACCATAAACCACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTGGTTTATGGTGATTGCATCTCGAGATGCAATCACCATAAACCACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTGGTTTATGGTGATTGCATCTCGAGATGCAATCACCATAAACCACG-3'

Other clones with same target sequence:

  1. TRCN0000053362