Construct: shRNA TRCN0000310582

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001681.3-1585s21c1
DNA Barcode:
CCCTTGGTTGTACTTCTGTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATP2A2 (488)
Gene Description:
ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2
Transcript:
RefSeq NM_001681.3 (NON-CURRENT)
Match location:
Position 1585 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000310582)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTTGGTTGTACTTCTGTTA
Hairpin Sequence:
5'-CCGG-CCCTTGGTTGTACTTCTGTTA-CTCGAG-TAACAGAAGTACAACCAAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTTGGTTGTACTTCTGTTACTCGAGTAACAGAAGTACAACCAAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTTGGTTGTACTTCTGTTACTCGAGTAACAGAAGTACAACCAAGGG-3'

Other clones with same target sequence:

  1. TRCN0000038531