Construct: shRNA TRCN0000310608

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014251.2-1870s21c1
DNA Barcode:
GCGGAGTGATAGACTGCTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC25A13 (10165)
Gene Description:
solute carrier family 25 member 13
Transcript:
RefSeq NM_014251.2 (NON-CURRENT)
Match location:
Position 1870 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000310608)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGGAGTGATAGACTGCTTTA
Hairpin Sequence:
5'-CCGG-GCGGAGTGATAGACTGCTTTA-CTCGAG-TAAAGCAGTCTATCACTCCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGGAGTGATAGACTGCTTTACTCGAGTAAAGCAGTCTATCACTCCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGGAGTGATAGACTGCTTTACTCGAGTAAAGCAGTCTATCACTCCGC-3'

Other clones with same target sequence:

  1. TRCN0000044068