Construct: shRNA TRCN0000310642

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001005739.1-1956s21c1
DNA Barcode:
GCCATGATCGAGCTGTCAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
VPS54 (51542)
Gene Description:
VPS54 subunit of GARP complex
Transcript:
RefSeq NM_001005739.1 (NON-CURRENT)
Match location:
Position 1956 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000310642)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATGATCGAGCTGTCAAAT
Hairpin Sequence:
5'-CCGG-GCCATGATCGAGCTGTCAAAT-CTCGAG-ATTTGACAGCTCGATCATGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATGATCGAGCTGTCAAATCTCGAGATTTGACAGCTCGATCATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATGATCGAGCTGTCAAATCTCGAGATTTGACAGCTCGATCATGGC-3'

Other clones with same target sequence:

  1. TRCN0000118365