Construct: shRNA TRCN0000310720

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018212.4-1225s21c1
DNA Barcode:
TGGGAGAGAGAGCGCAGAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ENAH (55740)
Gene Description:
ENAH actin regulator
Transcript:
RefSeq NM_018212.4 (NON-CURRENT)
Match location:
Position 1225 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000310720)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGGAGAGAGAGCGCAGAATA
Hairpin Sequence:
5'-CCGG-TGGGAGAGAGAGCGCAGAATA-CTCGAG-TATTCTGCGCTCTCTCTCCCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGGAGAGAGAGCGCAGAATACTCGAGTATTCTGCGCTCTCTCTCCCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGGAGAGAGAGCGCAGAATACTCGAGTATTCTGCGCTCTCTCTCCCA-3'

Other clones with same target sequence:

(none)