Construct: shRNA TRCN0000310901

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009007.2-833s21c1
DNA Barcode:
ATCAGCGAGCCTTCGCATTTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rac1 (19353)
Gene Description:
RAS-related C3 botulinum substrate 1
Transcript:
RefSeq NM_009007.2 (CURRENT)
Match location:
Position 833 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000310901)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATCAGCGAGCCTTCGCATTTG
Hairpin Sequence:
5'-CCGG-ATCAGCGAGCCTTCGCATTTG-CTCGAG-CAAATGCGAAGGCTCGCTGAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATCAGCGAGCCTTCGCATTTGCTCGAGCAAATGCGAAGGCTCGCTGATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATCAGCGAGCCTTCGCATTTGCTCGAGCAAATGCGAAGGCTCGCTGAT-3'

Other clones with same target sequence:

(none)