Construct: shRNA TRCN0000310916

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008055.4-2331s21c1
DNA Barcode:
CAGATAGCAAAGTGATCTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fzd4 (14366)
Gene Description:
frizzled class receptor 4
Transcript:
RefSeq NM_008055.4 (CURRENT)
Match location:
Position 2331 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000310916)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGATAGCAAAGTGATCTATA
Hairpin Sequence:
5'-CCGG-CAGATAGCAAAGTGATCTATA-CTCGAG-TATAGATCACTTTGCTATCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGATAGCAAAGTGATCTATACTCGAGTATAGATCACTTTGCTATCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGATAGCAAAGTGATCTATACTCGAGTATAGATCACTTTGCTATCTG-3'

Other clones with same target sequence:

(none)