Construct: shRNA TRCN0000310933

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007923.2-1715s21c1
DNA Barcode:
CGTCTGCAGTGCTCGTGATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Elk4 (13714)
Gene Description:
ELK4, member of ETS oncogene family
Transcript:
RefSeq NM_007923.2 (CURRENT)
Match location:
Position 1715 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000310933)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTCTGCAGTGCTCGTGATTT
Hairpin Sequence:
5'-CCGG-CGTCTGCAGTGCTCGTGATTT-CTCGAG-AAATCACGAGCACTGCAGACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTCTGCAGTGCTCGTGATTTCTCGAGAAATCACGAGCACTGCAGACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTCTGCAGTGCTCGTGATTTCTCGAGAAATCACGAGCACTGCAGACG-3'

Other clones with same target sequence:

(none)