Construct: shRNA TRCN0000311011

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009239.2-889s21c1
DNA Barcode:
TGTAACAACCCTACCAATTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sp4 (20688)
Gene Description:
trans-acting transcription factor 4
Transcript:
RefSeq NM_009239.2 (NON-CURRENT)
Match location:
Position 889 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000311011)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGTAACAACCCTACCAATTAA
Hairpin Sequence:
5'-CCGG-TGTAACAACCCTACCAATTAA-CTCGAG-TTAATTGGTAGGGTTGTTACA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTAACAACCCTACCAATTAACTCGAGTTAATTGGTAGGGTTGTTACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTAACAACCCTACCAATTAACTCGAGTTAATTGGTAGGGTTGTTACA-3'

Other clones with same target sequence:

  1. TRCN0000425976