Construct: shRNA TRCN0000311096

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010931.3-732s21c1
DNA Barcode:
CTGTAGCTCCAGTGCCGTTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Uhrf1 (18140)
Gene Description:
ubiquitin-like, containing PHD and RING finger domains, 1
Transcript:
RefSeq NM_010931.3 (CURRENT)
Match location:
Position 732 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000311096)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGTAGCTCCAGTGCCGTTAA
Hairpin Sequence:
5'-CCGG-CTGTAGCTCCAGTGCCGTTAA-CTCGAG-TTAACGGCACTGGAGCTACAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGTAGCTCCAGTGCCGTTAACTCGAGTTAACGGCACTGGAGCTACAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGTAGCTCCAGTGCCGTTAACTCGAGTTAACGGCACTGGAGCTACAG-3'

Other clones with same target sequence:

(none)