Construct: shRNA TRCN0000311201

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015805.2-1376s21c1
DNA Barcode:
TCCCATAAGTCTGCGAGTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Atp9b (50771)
Gene Description:
ATPase, class II, type 9B
Transcript:
RefSeq NM_015805.2 (NON-CURRENT)
Match location:
Position 1376 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000311201)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCCATAAGTCTGCGAGTAAA
Hairpin Sequence:
5'-CCGG-TCCCATAAGTCTGCGAGTAAA-CTCGAG-TTTACTCGCAGACTTATGGGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCCATAAGTCTGCGAGTAAACTCGAGTTTACTCGCAGACTTATGGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCCATAAGTCTGCGAGTAAACTCGAGTTTACTCGCAGACTTATGGGA-3'

Other clones with same target sequence:

(none)