Construct: shRNA TRCN0000311239

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173865.2-2077s21c1
DNA Barcode:
GTCTTCACACCTGTGATTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc41a1 (98396)
Gene Description:
solute carrier family 41, member 1
Transcript:
RefSeq NM_173865.2 (NON-CURRENT)
Match location:
Position 2077 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000311239)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCTTCACACCTGTGATTAAT
Hairpin Sequence:
5'-CCGG-GTCTTCACACCTGTGATTAAT-CTCGAG-ATTAATCACAGGTGTGAAGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCTTCACACCTGTGATTAATCTCGAGATTAATCACAGGTGTGAAGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCTTCACACCTGTGATTAATCTCGAGATTAATCACAGGTGTGAAGAC-3'

Other clones with same target sequence:

(none)