Construct: shRNA TRCN0000311392

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172133.1-1253s21c1
DNA Barcode:
GTTCACCTCAGCCCTAGTTGT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Adap2 (216991)
Gene Description:
ArfGAP with dual PH domains 2
Transcript:
RefSeq NM_172133.1 (CURRENT)
Match location:
Position 1253 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000311392)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTCACCTCAGCCCTAGTTGT
Hairpin Sequence:
5'-CCGG-GTTCACCTCAGCCCTAGTTGT-CTCGAG-ACAACTAGGGCTGAGGTGAAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTCACCTCAGCCCTAGTTGTCTCGAGACAACTAGGGCTGAGGTGAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTCACCTCAGCCCTAGTTGTCTCGAGACAACTAGGGCTGAGGTGAAC-3'

Other clones with same target sequence:

(none)