Construct: shRNA TRCN0000311411

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013671.3-769s21c1
DNA Barcode:
GCTTACTACCTTCAGTATAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sod2 (20656)
Gene Description:
superoxide dismutase 2, mitochondrial
Transcript:
RefSeq NM_013671.3 (CURRENT)
Match location:
Position 769 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000311411)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTACTACCTTCAGTATAAA
Hairpin Sequence:
5'-CCGG-GCTTACTACCTTCAGTATAAA-CTCGAG-TTTATACTGAAGGTAGTAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTACTACCTTCAGTATAAACTCGAGTTTATACTGAAGGTAGTAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTACTACCTTCAGTATAAACTCGAGTTTATACTGAAGGTAGTAAGC-3'

Other clones with same target sequence:

(none)