Construct: shRNA TRCN0000311517

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010313.1-488s21c1
DNA Barcode:
CTTGTGGTGGTCTGGATAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gnb5 (14697)
Gene Description:
guanine nucleotide binding protein (G protein), beta 5
Transcript:
RefSeq NM_010313.1 (NON-CURRENT)
Match location:
Position 488 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000311517)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTGTGGTGGTCTGGATAATA
Hairpin Sequence:
5'-CCGG-CTTGTGGTGGTCTGGATAATA-CTCGAG-TATTATCCAGACCACCACAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTGTGGTGGTCTGGATAATACTCGAGTATTATCCAGACCACCACAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTGTGGTGGTCTGGATAATACTCGAGTATTATCCAGACCACCACAAG-3'

Other clones with same target sequence:

(none)