Construct: shRNA TRCN0000311604

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001431.3-2126s21c1
DNA Barcode:
TAGTGAACTCAAGCGCAATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EPB41L2 (2037)
Gene Description:
erythrocyte membrane protein band 4.1 like 2
Transcript:
RefSeq NM_001431.3 (NON-CURRENT)
Match location:
Position 2126 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000311604)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAGTGAACTCAAGCGCAATTT
Hairpin Sequence:
5'-CCGG-TAGTGAACTCAAGCGCAATTT-CTCGAG-AAATTGCGCTTGAGTTCACTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAGTGAACTCAAGCGCAATTTCTCGAGAAATTGCGCTTGAGTTCACTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAGTGAACTCAAGCGCAATTTCTCGAGAAATTGCGCTTGAGTTCACTA-3'

Other clones with same target sequence:

(none)